Yi Tan, Shukui Qin, Xin Hou, Xiujuan Qian, Jun Xia, Yumei Li, Rui Wang, Changjie Chen, Qingling Yang, Lucio Miele, Qiong Wu and Zhiwei Wang Pages 81 - 87 ( 7 )
Background: Hepatocellular carcinoma (HCC) has high mortality partly due to acquiring drug resistance during chemotherapy treatment. Therefore, it is necessary to explore the underlying mechanism of drug resistance.
Methods: We used 2-DE and MALDI-TOF-MS analysis to explore the possible molecular insight into 5-FU resistance in HCC. The differentially expressed proteins were validated by Western blot analysis.
Results: We identified 102 unique proteins including p16, maspin, PRDX6, PSMB7, MYL6, PHB, and HSP27 with alteration in SMMC- 7721/5-FU. Furthermore, down-regulation of PRDX6 and PSMB7 enhanced SMMC-7721/5-FU cells to 5-FU sensitivity.
Conclusions: Our study suggests that targeting drug resistant genes such as PRDX6 and PSMB7 could be a novel approach to overcome 5-FU resistance in HCC cells.
Hepatocellular carcinoma, chemoresistance, 5-FU, PRDX6, PMSB7.
, , , , , , , , , , , Department of Medical Oncology, First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, China.