Frank T. Peters*, Dirk K. Wissenbach, Francesco Paolo Busardo, Emilia Marchei and Simona Pichini Pages 5455 - 5467 ( 13 )
Background: In the field of forensic toxicology, the quality of analytical methods is of great importance to ensure the reliability of results and to avoid unjustified legal consequences. A key to high quality analytical methods is a thorough method development.
Methods: The presented article will provide an overview on the process of developing methods for forensic applications.
Results: This includes the definition of the method’s purpose (e.g. qualitative vs quantitative) and the analytes to be included, choosing an appropriate sample matrix, setting up separation and detection systems as well as establishing a versatile sample preparation.
Conclusion: Method development is concluded by an optimization process after which the new method is subject to method validation.
Forensic toxicology, method development, hyphenated methods, systematic toxicological analysis, analytical methods, forensic applications.
Institute of Forensic Medicine, Jena University Hospital, 07740 Jena, Institute of Forensic Medicine, Jena University Hospital, 07740 Jena, Unit of Forensic Toxicology (UoFT) Department of Anatomical, Histological, Forensic and Orthopedic Sciences Sapienza University of Rome, National Centre On Addiction And Doping, Istituto Superiore di Sanita, Rome, National Centre On Addiction And Doping, Istituto Superiore di Sanita, Rome