Rodríguez Fonseca Rolando Alberto, Rodrigues Joao, Muñoz-Fernández María de los Angeles, Martínez Muñoz Alberto, Fragoso Vázquez Manuel Jonathan* and Correa Basurto José Pages 3076 - 3083 ( 8 )
Nanomedicine is the application of nanotechnology to medicine. This field is related to the study of nanodevices and nanomaterials applied to various medical uses, such as in improving the pharmacological properties of different molecules. Dendrimers are synthetic nanoparticles whose physicochemical properties vary according to their chemical structure. These molecules have been extensively investigated as drug nanocarriers to improve drug solubility and as sustained-release systems. New therapies such as gene therapy and the development of nanovaccines can be improved by the use of dendrimers. The biophysical and physicochemical characterization of nucleic acid/peptide-dendrimer complexes is crucial to identify their functional properties prior to biological evaluation. In that sense, it is necessary to first identify whether the peptide-dendrimer or nucleic aciddendrimer complexes can be formed and whether the complex can dissociate under the appropriate conditions at the target cells. In addition, biophysical and physicochemical characterization is required to determine how long the complexes remain stable, what proportion of peptide or nucleic acid is required to form the complex or saturate the dendrimer, and the size of the complex formed. In this review, we present the latest information on characterization systems for dendrimer-nucleic acid, dendrimer-peptide and dendrimer-drug complexes with several biotechnological and pharmacological applications.
Nanovaccines, gene therapy, dendrimers, peptides, nucleic acids, functional properties characterization.
Laboratorio de Modelado Molecular y Diseño de Fármacos, Escuela Superior de Medicina-Instituto Politécnico Nacional, Mexico City 11340, CQM-Centro de Quimica da Madeira, MMRG, Universidade da Madeira, Campus da Penteada 9020-105, Funchal, Laboratorio InmunoBiología Molecular and Plataforma de Laboratorio, Hospital General Universitario Gregorio Marañón (HGUGM), Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM), Spanish HIV-HGM BioBank, Madrid, Spain; Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Laboratorio de Modelado Molecular y Diseño de Fármacos, Escuela Superior de Medicina-Instituto Politécnico Nacional, Mexico City 11340, Departamento de Química Orgánica, Escuela Nacional de Ciencias Biológicas-Instituto Politécnico Nacional, Laboratorio de Modelado Molecular y Diseño de Fármacos, Escuela Superior de Medicina-Instituto Politécnico Nacional, Mexico City 11340