Mohammad Darvishi*, Shahrzad Farahani and Azadeh Haeri Pages 1 - 6 ( 6 )
Background: Pulmonary infections are an increasing problem in individuals and current therapies are lacking. Liposomes are spherical lipidic vesicles composed of phospholipid and cholesterol. Liposomes have numerous advantages, such as biodegradability, biocompatibility, non-immunogenicity, lack of toxicity, controlled release properties and high stability. Objective: This work was carried out to construct a novel liposomal moxifloxacin formulation and examine its antimicrobial effects against Pseudomonas aeruginosa and Staphylococcus aureus.
Methods: The liposomal moxifloxacin formulation was prepared by the thin film hydration method. The bilayer was composed of cholesterol and phospholipid at 30:70 molar ratio. To prepare cationic liposomes 5% cationic agent (CTAB) was added. The liposomes were sized down with bath sonication technique. The liposomal characterizations were tested regarding vesicle size, surface charge and drug encapsulation efficacy. Microdilution method was used to determine the Minimum Inhibitory Concentration (MIC) for Pseudomonas aeruginosa and Staphylococcus aureus of free drug, neutral and cationic moxifloxacin liposomes.
Results: The size of liposomes was 50-70 nm. The zeta potential of neutral and cationic vesicles was ~ 0 and +22 mV. The MIC values to Pseudomonas aeruginosa of free drug, neutral and cationic moxifloxacin liposomes were 10, 5 and 2.5, respectively. The MICs against Staphylococcus aureus of free drug, neutral and cationic moxifloxacin liposomes were 1, 1 and 0.5, respectively.
Conclusion: This study demonstrates that the encapsulation of moxifloxacin into liposomes (especially cationic vesicles) could enhance antimicrobial properties.
Nanoliposomes, Moxifloxacin, Antimicrobial effect, Infection, liposomes, Minimum Inhibitory Concentration (MIC).
lnfectious Diseases and Tropical Medicine Research Center (IDTMRC), AJA University of Medical Sciences, Tehran, School of Medicine, Azad University, Tehran, Department of Pharmaceutics, School of Pharmacy, Pharmaceutical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran