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Probucol-induced hERG Channel Reduction can be Rescued by Matrine and Oxymatrine in vitro

Author(s):

Yuan-qi Shi, Pan Fan, Guo-cui Zhang, Yu-hao Zhang, Ming-zhu Li, Fang Wang and Bao-xin Li*   Pages 1 - 7 ( 7 )

Abstract:


The human ether-a-go-go-related gene (hERG) potassium channel is the rapidly activating component of cardiac delayed rectifier potassium current (IKr), which is a crucial determinant of cardiac repolarization. The reduction of hERG current is commonly believed to cause Long QT Syndrome (LQTs). Probucol, a cholesterol-lowering drug, induces LQTs by inhibiting the expression of the hERG channel. Unfortunately, there is currently no effective therapeutic method to rescue probucol-induced LQTs. In this study, we demonstrated that 1 μM matrine and oxymatrine could rescue the hERG current and hERG surface expression inhibited by probucol. In addition, matrine and oxymatrine significantly shortened the prolonged action potential duration induced by probucol in neonatal cardiac myocytes. We proposed a novel mechanism underlying the probucol induced decrease in the expression of transcription factor Specificity protein 1 (Sp1), which is an established transactivator of the hERG gene. We also demonstrated that matrine and oxymatrine were able to upregulate Sp1 expression which may be one of the possible mechanisms that by which matrine and oxymatrine rescued probucol-induced hERG channel deficiency. Therefore, these results demonstrate that matrine and oxymatrine could rescue probucol-induced hERG deficiency, which may lead to potentially effective clinical therapeutic drugs for treating cardiotoxicity in the future.

Keywords:

Probucol, human ether-a-go-go-related gene, Matrine, Oxymatrine, Specificity protein 1

Affiliation:

Department of Cardiology, The First Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, Nangang District, Harbin 150001, Department of Ophthalmology, the Second Affiliated Hospital of Harbin Medical University, No. 148 Baojian Road, Nangang District, Harbin 150081, Department of Pharmacology, Harbin Medical University, No. 157 Baojian Road, Harbin, 150086, Department of Pharmacology, Harbin Medical University, No. 157 Baojian Road, Harbin, 150086, Department of Pharmacology, Harbin Medical University, No. 157 Baojian Road, Harbin, 150086, Department of Pharmacology, Harbin Medical University, No. 157 Baojian Road, Harbin, 150086, Department of Pharmacology, Harbin Medical University, No. 157 Baojian Road, Harbin, 150086



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