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Biotin and Glutathione Targeting of Solid Nanoparticles to Cross Human Brain Endothelial Cells

[ Vol. 23 , Issue. 28 ]

Author(s):

Szilvia Veszelka, Maria Meszaros, Lorand Kiss, Zoltan Kota, Tibor Pali, Zsofia Hoyk, Zsolt Bozso, Livia Fulop, Andras Toth, Gabor Rakhely and Maria A. Deli*   Pages 4198 - 4205 ( 8 )

Abstract:


Background: The blood-brain barrier restricts drug penetration to the central nervous system. Targeted nanocarriers are new potential tools to increase the brain entry of drugs. Ligands of endogenous transporters of the blood-brain barrier can be used as targeting vectors for brain delivery of nanoparticles.

Objective: We tested biotin-labeled solid nanoparticles for the first time and compared to biotinylated glutathione- labeled nanoparticles in brain endothelial cells.

Method: Neutravidin coated fluorescent polystyrene nanoparticles were derivatized with biotin and biotinylated glutathione. As a human in vitro blood-brain barrier model hCMEC/D3 brain endothelial cells were used. Cell viability by MTT test, uptake and transfer of the nanoparticles across the endothelial monolayers were measured. The uptake of the nanoparticles was visualized by confocal microscopy.

Results: The tested nanoparticles caused no change in cell viability. The uptake of biotin- and glutathione-labeled nanoparticles by brain endothelial cells was time-dependent and significantly higher compared to non-labeled nanoparticles. The penetration of the glutathione-labeled nanoparticles across the endothelial monolayer was higher than the biotin-targeted ones. Biotin- and glutathione-targeted nanoparticles were visualized in hCMEC/D3 cells. We verified that hCMEC/D3 express mRNA for sodium-dependent multivitamin transporter (SMVT/SLC5A6) responsible for the blood-brain barrier transport of biotin.

Conclusion: Biotin as a ligand increased the uptake and the transfer of nanoparticles across brain endothelial cells. Biotinylated glutathione could further increase nanoparticle permeability through endothelial monolayers supporting its use as a brain targeting vector.

Keywords:

Biotin, blood-brain barrier, brain endothelial cell, glutathione, solute carriers, solid nanocarriers, targeted nanoparticle.

Affiliation:

Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Temesvari krt. 62, H-6726 Szeged, Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Temesvari krt. 62, H-6726 Szeged, Department of Pathophysiology, University of Szeged, Semmelweis u. 1, H-6701 Szeged, Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Temesvari krt. 62, H-6726 Szeged, Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Temesvari krt. 62, H-6726 Szeged, Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Temesvari krt. 62, H-6726 Szeged, Department of Medical Chemistry, University of Szeged, Dom ter 8, H-6720 Szeged, Department of Medical Chemistry, University of Szeged, Dóm tér 8, H-6720 Szeged, Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Temesvari krt. 62, H-6726 Szeged, Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Temesvari krt. 62, H-6726 Szeged, Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Temesvari krt. 62, H-6726 Szeged



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