Anandarajagopal Kalusalingam*, Abdullah Khan, Mahibub Mahamadsa Kanakal, Syed Atif Abbas, Chiau Ming Long, Tazneem B., Ching Siang Tan, Abubakar Salam Bawazir, Abdul Razak T. K. and Roshan S Pages 1 - 15 ( 15 )
Background: This study explores the formulation and effectiveness of a Rhodiola rosea phytosomal gel for enhancing wound healing.
Objective: The ethanolic extract of Rhodiola rosea roots, rich in bioactive compounds like alkaloids and flavonoids, was optimized into a phytosomal complex to improve absorption and dermal retention.
Methods: Characterization through GC-MS revealed compounds, such as 2-Heptadecenal and Bicyclo[ 4.1.0]Heptane and 7-Pentyl. FTIR confirmed the successful encapsulation within the phospholipid bilayer, while SEM showed smooth, spherical particles.
Results: The Box-Behnken design optimized formulation parameters, achieving high yield (92.64%), small particle size (355 nm), and high entrapment efficiency (93.98%). In vitro release studies displayed a consistent release profile, aligning with Zero-order and Hixson-Crowell models. In vivo evaluation on Wistar rats showed that the phytosomal gel significantly enhanced wound healing, achieving 98.16% wound reduction by day 14, compared to 95.17% for R. rosea extract and 97.13% for standard treatment. Histopathological analysis demonstrated complete tissue regeneration and well-organized collagen fibers in the phytosomal gel group.
Conclusion: This research highlights the potential of Rhodiola rosea phytosomal gel as an effective wound healing therapy, with future studies suggested for extended stability tests and human skin permeation studies.
Rhodiola rosea, phytosomal gel, wound healing, extraction, Box-Behnken design, in vivo evaluation.