Irfan Ahmad, Mushtaq Ahmad Mir, Saurabh Srivastava*, Ali A. Shati, Serag Eldin I. Elbehairi, Safia Irfan, Mohammed Abohashrh, Nazima Nisar, Nasreena Bashir and Pragya Srivastava Pages 69 - 79 ( 11 )
Background: Infectious diseases constantly represent the source of sickness as well as mortality in human beings. Herbal applications in human life through using plants for antibacterial and anticancer activity have shown the potential medicinal outcome.
Objectives: To evaluate the antibacterial and anticancer activities of the crude extract of Matricaria aurea.
Materials and Methods: The antibacterial activity of the crude flowers of M. aurea extract was examined against reference and clinical bacterial strains by agar well diffusion method. Minimum inhibitory concentrations and minimum bactericidal concentrations were determined by micro broth dilution assays using MH broth. Herbal extract was employed over human breast adenocarcinoma cell line (MCF-7), hepatocellular carcinoma cell line (HepG-2) and colorectal adenocarcinoma cell line (HCT-116) to optimize cancer cells proliferation by SRB assay.
Results: The data has shown that the extract from M. aurea had significant antimicrobial activity against the tested microorganisms. The plant extract showed higher antibacterial activity against the reference strain of Streptococcus pyogenes. The MIC and MBC varied between 0.38-12.5 mg/ml and 3.1-200 mg/ml respectively. Synergy study elucidated the significant bacteriostatic effect of M. aurea extract on S. aureus and S. saprophyticus. The data of SRB assay deliver the potential anticancer activity through cell death.
Conclusion: This study delivers innovative information that M. aurea possessed excellent bio-activities against pathogenic microbes and cancer cells, which drive attention for further research to explore the active components responsible for biological efficacies.
M. aurea, anticancer activity, antibacterial activity, antibiotics, inhibition zone, crude extract.
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha, Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha, Department of Pharmaceutics, Era College of Pharmacy, Era University, Lucknow, UP, Department of Biology, College of Science, King Khalid University, Abha, Department of Biology, College of Science, King Khalid University, Abha, Department of Biosciences, Faculty of Science, Integral University, Lucknow, Department of Basic Medical Sciences, College of Applied Medical Sciences, King Khalid University, Abha, Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha, Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha, Division of Research & Development, Narmix Labs Ltd, Chennai