Zhibo Sun, Fei Wu*, Yue Yang, Feng Liu, Fengbo Mo, Jin Chen, Guangyong Wang and Bo Zhang* Pages 4806 - 4812 ( 7 )
Background: MicroRNAs have recently been recognized to be engaged in the development of bone diseases.
Objective: This study was performed to elucidate the effects of miR-144-3p on proliferation and osteogenesis of mesenchymal stem cells (MSCs) from the patients with steroid-associated osteonecrosis (ONFH) and its related mechanism. Method: The expression level of miR-144-3p in the MSCs from the proximal femur of the patients was examined by Real-time PCR. The cell proliferation ability was assayed by MTT. The differentiation ability of MSCs was assayed by Alizarin Red S (ARS) staining. The interaction between miR-144-3p and frizzled4 (FZD4) was investigated by Real-time PCR, western blot and luciferase reporter assay. Results: ONFH samples had the obviously high expression of miR-144-3p compared to the control. MiR-144-3p had a negative effect on the proliferation and osteogenesis of MSCs. Via targeting FZD4, miR-144-3p decreased β-catenin nuclear translocation, the transcription of RUNX2 and COL1A1. Over-expression of FZD4 partially reversed miR-144-3p-induced decrease in the proliferation and osteogenesis of MSCs. Conclusion: MiR-144-3p might play an important role in the development of ONFH and might be used as a novel class of therapeutic targets for this disease.MiR-144-3p, FZD4, Steroid-associated osteonecrosis, Mesenchymal stem cells, ONFH, Real-time PCR.
Department of Orthopedics, Renmin Hospital, Wuhan University, Wuhan, Department of Orthopedics, Renmin Hospital, Wuhan University, Wuhan, Department of Orthopedics, Renmin Hospital, Wuhan University, Wuhan, Department of Orthopedics, Renmin Hospital, Wuhan University, Wuhan, Department of Orthopedics, The First Affiliated Hospital of Nanchang University, Nanchang, Department of Orthopedics, Yiling Hospital, Yichang, Department of Orthopedics, Yiling Hospital, Yichang, Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan